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人参黄芩配伍对多发性脑梗塞性痴呆模型大鼠脑能量的影响
Effects of compatibitily of Renshen and Huangqin on brain energy in multi-infarct dementia rats induced by thrombus injection
投稿时间:2018-12-09  修订日期:2018-12-27
DOI:
中文关键词:  多发性脑梗塞性痴呆  人参黄芩配伍 脑能量  线粒体
英文关键词:Multi-infarct  Dementia, Compatibility  of Renshen  and Huangqin, Energy , Mitochondria
基金项目:
作者单位E-mail
付颖 成都中医药大学药学院 fuying1222@aliyun.com 
夏鹏 成都中医药大学药学院  
高丽娟 成都中医药大学药学院  
文跃强 成都中医药大学中医脑病药物整合转化研究所 wenyueqiang@163.com 
徐世军 成都中医药大学药学院  
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中文摘要:
      目的:考察人参黄芩配伍对多发性脑梗塞性痴呆模型大鼠学习记忆功能的影响,并从脑能量角度探讨其作用机制。方法:采用微血栓栓塞法制备多发性脑梗塞性大鼠痴呆模型,采用Morris水迷宫评价其学习记忆能力;HE染色法观察海马CA1区病理学形态学;HPLC法测定脑组织ATP、ADP和AMP含量;流式细胞术检测大鼠海马线粒体肿胀及膜电位变化。结果:与模型组比较,人参黄芩配伍组逃避潜伏期显著缩短(P<0.05),穿越平台次数明显增加(P<0.05);海马CA1区椎体细胞排列紊乱和细胞数目减少明显改善(P<0.05);脑能荷值显著提高(P<0.05),线粒体肿胀度显著减轻,膜电位水平明显增高(P<0.05)。结论:人参黄芩配伍具有改善多发性脑梗塞性痴呆模型大鼠学习记忆功能的作用,保护线粒体损伤,改善脑能量进而保护神经细胞是其主要作用机制之一。
英文摘要:
      Objective: To investigate the effect of compatibility of Renshen and Huangqin (CRH) on learning and memory ability in multi-infarct dementia (MID) model rats and to explore the mechanism from the perspective of brain energy.Methods: We established MID rat model by thrombus injection, and learning and memory ability of MID rats was evaluated by Morris water maze; HE staining was used to observe the pathological changesSin hippocampal CA1 region of rats; the contents of ATP, ADP and AMP in brain tissues were determined by high-performance liquid chromatography; flow cytometry was used to detect the changes of mitochondrial swelling and mitochondrial membrane potential in hippocampus of rats. Results: Compared with the rats in model group, the escape latency was significantly shortened in both high and low dose groups (P < 0.05), SThe times of crossing the platform were increased in both high and low dose groups (P < 0.05), The disorder of cell arrangement and loss of number of cell layers were significantly improved in high and low dose groups(P < 0.05), the degree of mitochondria swelling was significantly reduced in low dose group (P < 0.05), the decreasing trend of mitochondrial membrane potential was improved (P < 0.05), and the energy charge of brain tissue was increased in high dose group rats (P < 0.05).Conclusion:CRH improved the cognitive decline and increased the concentration of energy charge in MID rats Which was relation to protect mitochondrial damage and improve brain energy furtherly protect neurons.
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