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参芪益智颗粒对Aβ1-42双侧海马注射AD模型大鼠PI3K/AKT信号通路的影响
Effect of Shenqi Yizhi Granules on PI3K/AKT Signaling Pathway in Aβ1-42 Bilateral Injection of AD Model
投稿时间:2018-12-09  修订日期:2018-12-11
DOI:
中文关键词:  参芪益智颗粒 Aβ1-42 PI3K/AKT信号通路
英文关键词:Shenqi  Yizhi granules  Aβ1-42 PI3K/AKT Signaling Pathway
基金项目:
作者单位E-mail
秦莉霞 成都中医药大学药学院 1032532815@qq.com 
吴倩 成都中医药大学药学院  
夏鹏 成都中医药大学药学院  
高丽娟 成都中医药大学药学院  
徐世军 成都中医药大学药学院 xushijun@cdutcm.edu.cn 
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中文摘要:
      目的 探讨参芪益智颗粒对Aβ1-42双侧海马注射AD模型大鼠PI3K/AKT信号通路的影响。方法 采用双侧海马注射Aβ1-42建立AD大鼠模型,参芪益智颗粒干预60d,Morris水迷宫检测大鼠学习记忆功能,HE染色观察大鼠海马CA3区病理形态变化,免疫组化检测海马PI3K,AKT蛋白表达,RT-PCR检PI3KmRNA、AKT mRNA的相对表达。结果 与空白组相比,模型组动物逃避潜伏期明显延长、进入平台次数、穿越平台象限时间及百分比明显减少(P<0.05);同时海马CA3区椎体细胞排列紊乱,神经元出现明显病变;海马PI3K/AKT在蛋白及基因水平上均呈明显抑制状态(P<0.05)。经参芪益智颗粒干预后,该模型大鼠逃避潜伏期明显缩短、进入平台次数、穿越平台象限时间明显增加(P<0.05)。结论 参芪益智颗粒可改善Aβ1-42双侧海马注射AD模型大鼠学习认知功能,其机制可能与激活脑内PI3K/AKT信号通路有关。
英文摘要:
      Objective To investigate the effect of Shenqi Yizhi Granules on PI3K/AKT signaling pathway in Aβ1-42 bilateral hippocampal injection of AD model rats. Methods A rat model of AD was established by bilateral hippocampus injection of Aβ1-42. Shenqi Yizhi granules were used for 60 days. Morris water maze was used to detect the learning and memory function of each rat. HE staining was used to observe the pathological changes of rat hippocampal CA3 area. Immunohistochemistry The expression of PI3K and AKT protein in hippocampus was detected by RT-PCR. The relative expression levels of PI3K mRNA and AKT mRNA were detected by RT-PCR. Results fr.Compared with the blank group, the escape latency of the model group was significantly prolonged, the number of entering the platform, the time and percentage of crossing the platform quadrant decreased significantly (P<0.05). At the same time, the vertebral cells in the hippocampal CA3 area were disordered and the neurons showed obvious lesions. PI3K/AKT showed significant inhibition at both protein and gene levels (P<0.05). After intervention with Shenqi Yizhi Granule, the escape latency of the model rats was significantly shortened, the number of entering the platform and the time of crossing the platform quadrant were significantly increased (P<0.05). Conclusion Shenqi Yizhi Granules can improve the cognitive function of Aβ1-42 bilateral hippocampus injection in AD rats, and its mechanism may be related to activation of PI3K/AKT signaling pathway in brain.
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