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中药标准品对人肺癌耐药细胞A549/DDP的逆转效果研究
Research on the Reversal effects in Cisplatin-resistant Human Lung Adenocarcinoma Cell Line A549/DDP by Standard Substances from Traditional Chinese Medicine
投稿时间:2017-12-28  修订日期:2018-01-22
DOI:
中文关键词:  中药标准品、Tariquidar、A549细胞、A549/DDP细胞、耐药
英文关键词:Standard Substances, Tariquidar, A549 Cell Line,A549/DDP Cell Line, Drug Resistance
基金项目:
作者单位E-mail
杨新阶 北京中医药大学 yms6986@163.com 
王婧筱 北京中医药大学  
王琳 北京中医药大学  
迟笑怡 北京中医药大学  
迟佳琦 北京中医药大学  
周天 北京中医药大学东方医院  
刘铜华 北京中医药大学中医养生学教育部重点实验室  
胡凯文 北京中医药大学东方医院 kaiwenh@163.com 
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中文摘要:
      目的:研究选定中药标准品对人肺腺癌耐顺铂细胞株A549/DDP的逆转效果。 方法:以CCK8法检测人肺腺癌细胞株A549及A549/DDP对顺铂的耐受水平;中药标准品对A549/DDP增殖的影响;工作浓度中药标准品逆转A549/DDP耐药的效果。梯度浓度顺铂作用A549和A549/DDP细胞24h后,加入CCK8孵育60min后读取450nm处吸光值,回归分析计算IC50。梯度浓度中药标准品作用A549/DDP细胞24h后,加入CCK8孵育60min后读取450nm处吸光值,计算IC5、IC10、IC50,并选定工作浓度。工作浓度中药标准品孵育A549/DDP细胞24h后更换梯度浓度顺铂孵育24h,加入CCK8孵育60min后读取450nm处吸光值,回归分析计算IC50。结果:A549及A549/DDP对顺铂的IC50分别为32.71±2.8μM和1040±30.1μM,故耐药指数为31.79。A549/DDP对川芎嗪、苦参碱耐受良好。盐酸小檗碱、北豆根碱(蝙蝠葛碱)和冬凌草甲素对A549/DDP细胞增殖均有一定的抑制作用,且存在明显的量效关系。其IC5作为逆转及工作浓度时,均能下调A549/DDP对顺铂的IC50(P<0.01)。结论:选定的中药标准品能有效逆转A549/DDP对顺铂的耐药性。
英文摘要:
      To investigate the reversal effects in A549/DDP by standard substances from Traditional Chinese Medicine. Methods: CCK8 was applied to assay the IC50S of both A549 and A549/DDP cell lines on DDP, the inhibitory effects of standard substances (SS) on A549/DDP cell line, the reversal effects of working concentrations of SS on A549/DDP cell line. DDP gradient concentration was incubated with both A549 and A549/DDP cell lines for 24h and CCK8 solution was added, O.D. absorption at 450nm was read after 60 min incubation. SS gradient concentration was incubated with A549/DDP cell line for 24h, and CCK8 assay was applied to calculate the IC5, IC10 and IC50 by nonlinear regressions and the IC5 was selected as the working concentrations which incubated with A549/DDP for 24h and followed by CCK8 assay the IC50. Results: The IC50s of A549 and A549/DDP cell lines were 32.71±2.8μM和1040±30.1μM respectively and the resistant index was 31.79. Tetramethylpyrazine and Matrine were well tolerated. Berberine hydrochloride, Dauricine, Oridomin exhibited dose-depend inhibitory effects on A549/DDP cell line. The working concentrations of them could effectively reverse the resistance of A549/DDP cell line to DDP. (P<0.01) Conclusion: Selected standard substances from Traditional Chinese Medicine were capable to reverse the resistance of A549/DDP cell line to DDP.
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