• 主管部门:
  • 中国科学院
  • 主办单位:
  • 中科院科技战略咨询研究院
  • 出版单位:
  • 世界科学技术杂志社
    科学出版社
  • 主编:
  • 陈凯先
  • 执行主编:
  • 胡镜清 陈士林 叶阳
  • 副主编:
  • (按姓氏笔划排列)王 伟 王 阶 王拥军 王喜军 孔令义 吕文亮 孙晓波 李 萍 李志刚 张卫东 陈立典 果德安 罗国安 赵军宁 徐宏喜 高秀梅 唐旭东 梁繁荣 葛金文
  • 名誉社长:
  • 徐伟宣
  • 社长:
  • 张志华
  • 编辑部主任:
  • 王 瑀
  • 编辑部:
  • 张娜娜 马雅静 方垚 王涵 何岸波 郭嫦娥
  • 责任译审:
  • 王晶
  • 国际刊号:
  • 1876-3553
  • 国内刊号:
  • 11-5699/R
  • 社址:
  • 北京市海淀区中关村东路55号思源楼1203
  • 邮编:
  • 100190
  • 电话:
  • 010-62616352
  • 网址:
  • www.wst.ac.cn
  • E-mail:
  • wst@casipm.ac.cn
  • 海外发行号:
  • M1644
  • 邮刊代号:
  • 2-534

金振口服液对脂多糖诱导大鼠急性肺损伤的实验研究
Experimental Study of Jinzhen Koufuye on Acute Lung Injury Induced by Lipopolysaccharide in Rats
投稿时间:2013-03-14  修订日期:2013-05-30
DOI:10.11842/wst.2013.09.018
中文关键词:  金振口服液 急性肺损伤 脂多糖 炎症细胞因子
英文关键词:Jinzhen Koufuye, acute lung injury, lipopolysaccharide, inflammatory cytokines
基金项目:科学技术部国家重大新药创制项目(2013ZX090402203):现代中药创新集群与数字制药技术平台,负责人:王振中。
作者单位
李素君 江苏康缘药业股份有限公司/中药制药过程新技术国家重点实验室 连云港 222001 
韩兆忠  
秦萍  
周军  
王振中  
萧伟  
摘要点击次数: 2900
全文下载次数: 4540
中文摘要:
      目的:探讨金振口服液(JZKFY)对脂多糖(LPS)致急性肺损伤(ALI)大鼠的影响。方法:实验动物随机分为正常组、模型组、阳性对照组、JZKFY 高、中、低3 个剂量(4.4、2.2、1.1 g·kg-1),各给药组连续灌胃给药7 天;末次给药后1 h,除正常组外,每鼠气管内滴注LPS(6 mg·kg-1)复制LPS 致ALI 大鼠模型,正常对照组给予等体积生理盐水,注射LPS 16 h 后麻醉大鼠,取肺,进行组织学观察,并检测肺通透性、肺组织MPO、MDA、SOD 活性、血清TNF-ɑ、IL-6、IL-1β的含量。结果:JZKFY 高、中、低剂量组均能降低肺通透指数;JZKFY 高、中剂量组能调节肺组织MPO、MDA、SOD 活性;JZKFY 高、中剂量组明显降低血清TNF-ɑ、IL-6、IL-1β的含量;JZKFY 高、中剂量组明显改善ALI 的肺组织病变。结论:JZKFY 对LPS 诱导的急性肺损伤具一定的改善作用,其机制可能与改善肺血管通透性,减轻肺内中性粒细胞聚集,提高抗氧化应激能力及下调炎症反应有关。
英文摘要:
      This study was aimed to explore effects of Jinzhen Koufuye (JZKFY) on acute lung injury (ALI) induced by lipopolysaccharide (LPS) in rats. Rats were randomly divided into the normal group, model group, positive control group, and three JZKFY groups with the drug dosage of 4.4, 2.2, 1.1 g·kg-1, respectively. Intragastric administration was given to animals for 7 consecutive days. One hour after the last administration, ALI model was induced by the injection of LPS (6 mg·kg-1), and then rats were killed at 16 h after saline (control) or LPS injection. Histological examinations were performed on the lungs. At the same time, the lung permeability index, activities of MPO, MDA and SOD in lung tissues, contents of TNF-ɑ, IL-6 and IL-1β in serum were measured, respectively. The results showed that JZKFY of high-, medium-, or low-dosage can significantly reduce JZKFY lung permeability index.JZKFY of high- and medium-dosage can obviously regulate activities of MPO, MDA and SOD in lung tissues, reduce contents of TNF-ɑ, IL-6 and IL-1β in serum, as well as significantly improve lung tissue lesions in ALI. It was concluded that JZKFY might improve ALI induced by LPS through inhibiting lung vascular permeability, reducing the lung neutrophil aggregation, improving the ability of antioxidative stress and lowering inflammatory response.
查看全文  查看/发表评论  下载PDF阅读器
关闭