目的 观察三仁汤对脾胃湿热型大鼠NLRP3、Caspase-1蛋白的调控及胃黏膜细胞焦亡的影响。 方法 将60只雄性SD大鼠随机分为正常组、模型组、三仁汤高、中、低剂量组、奥美拉唑组,每组各10只。根据前期的研究方法,利用高脂、高糖、湿热的条件，复制脾胃湿热证得模型,给予三仁汤干预,高、中、低剂量分别为16.4g/kg,8.2 g/kg ,4.1g/kg,奥美拉唑为3.5mg/kg。 HE染色观察胃黏膜病理变化,免疫印迹法检测大鼠胃黏膜细胞NLRP3、Caspase-1蛋白的表达。 结果 HE结果显示,三仁汤高、中、低剂量组以及奥美拉唑组均对胃黏膜细胞焦亡有所改善。免疫印迹的结果显示模型组大鼠NLRP3和Caspase-1高表达,三仁汤高、中、低剂量组下调NLRP3和Caspase-1蛋白的表达,具有统计学差异(P<0.05)。结论 三仁汤可能通过降低脾胃湿热大鼠的NLRP3、Caspase-1蛋白的表达,减少胃粘膜细胞焦亡,从而抑制胃粘膜炎症的爆发，从而防治脾胃湿热证模型大鼠，为临床治疗脾胃湿热证的胃痛提供了理论依据。
Objective To observe the effect of Sanren Decoction on the regulation of NLRP3 and Caspase-1 protein in spleen-stomach damp-heat type rats and the apoptosis of gastric mucosa cells. Methods Sixty male Sprague-Dawley rats were randomly divided into normal group, model group, Sanrentang high, middle and low dose groups, and omeprazole group, with 10 rats in each group. According to the previous research method,using the high-fat, high-sugar, hot-damp conditions， the model of spleen-stomach damp-heat syndrome was replicated and given the intervention of Sanren Decoction. The high, medium and low doses were 16.4g/kg, 8.2g/kg, 4.1g/kg, and omeprazole was 3.5mg/ Kg. The pathological changes of gastric mucosa were observed by HE staining. The expression of NLRP3 and Caspase-1 protein in rat gastric mucosa was detected by immunoblotting. Results HE results showed that the Sanrentang high, medium and low dose groups and the omeprazole group all improved the gastric mucosal cell coke. The results of immunoblotting showed that NLRP3 and Caspase-1 were highly expressed in the model group, and the expression of NLRP3 and Caspase-1 protein was down-regulated in the high, middle and low doses of Sanren Decoction, which was statistically significant (P<0.05). Conclusion Sanren Decoction may reduce the gastric mucosal cell inflammation by reducing the expression of NLRP3 and Caspase-1 protein in rats with spleen and stomach dampness and heat, thus inhibiting the outbreak of gastric mucosal inflammation，and preventing and treating spleen and stomach heat and dampness model rats. It also provides a theoretical basis for the clinical treatment of gastric pain of spleen and stomach dampness and heat syndrome.