BSTRACT OBJECTIVE: To evaluate the effect of Pandanus tectorius on weight loss and blood lipid-lowering in mice. METHODS: 7weeks old SPF male C57BL/6 mice were divided into normal group, high-fat high-sugar fed group. Mice in the normal group were fed ordinary fodder and others were fed high-fat high-sugar fodder. After 30 days, the mice in high-fat high-sugar fed group were selective divided into obesity model group and Pandanus tectorius fed group (drink three different concentration solutions instead of water).There mice raised for another 54 days. Body weights and food intakes were determined every week. At the end of the experiment, the body fat were weighted and the fat/body weight, body weight gains were calculated. The contents of CHO, TG, HDL, LDL in serum were measured. The liver tissue pathological slices by HE staining were also observed. RESULTS: Body weight, weight gain, fat/body weight ratio and serum lipid level in the obesity model group were significantly higher than those in the normal control group (p<0.01~0.05).The intervention group with 2.4mg/mL and 1.2mg/mL YBL solution could significantly reduce the weight gain trend of mice, reduce the final weight gain (p<0.01~0.05), reduce the serum CHO and LDL levels (p<0.01~0.05), and reduce the degree of liver tissue fat change. CONCLUSIONS: Pandanus tectorius extract can effectively inhibit obesity in mice induced by high-fat and high-sugar diets, reduce serum CHO and LDL levels, and improve the pathological morphology of liver tissues.