Objective: To comparatively perform qualitative and semi-quantitative analysis on the volatile constituents in roots, stems leaves and insect gells of Fissistigma oldhamii. Methods: The volatile components were analyzed by HS-GC-MS, and the obtained data were treated using XCMS-online and multivariate statistical analysis, including partial least squares discriminant analysis (PLS-DA) and orthogonal partial least squares discriminant analysis (OPLS-DA). The retention index (RI) method was using to confirm the result of qualitative analysis, and the semi-quantitative relative percentage content of each component was calculated with the relative fragment ion&quot; strength, which values of variable importance for the projection (VIP) are more than 1. Results: There were 41 components separated from four parts of F. oldhamii, including 19 common components in different parts. The roots, stems, leaves and insect galls were detected and had 24, 38, 35 and 25 compounds separately. Conclusion: The principal volatile constituents were olefins, aliphatic hydrocarbons and their derivatives, which were different in four parts. The results would provide basis for the further application, efficacy evaluation, scientific development of quality standard and utilization of F. oldhamii.