目的：观察电针“委中”穴后的血清对大鼠腰多裂肌卫星细胞(MSCs)增殖、胶原蛋白I(Collagen I)及基质金属蛋白酶2(MMP2)表达的影响,探讨电针“委中”穴促进多裂肌损伤后修复的作用机制。方法：25只SD大鼠随机分为正常组、模型组、电针组,每组8只。通过肌肉注射0.5%的布比卡因建立大鼠腰多裂肌损伤的动物模型,选取“委中”穴进行电针干预,正常组、模型组不予电针干预,第4d收集各组大鼠血清。原代培养MSCs并进行细胞鉴定,分别以对应血清培养24h,检测MSCs增殖、Collagen I及MMP2蛋白的表达。结果：与正常血清组比较,模型血清组MSCs增殖显著加快(P<0.01),Collagen I、MMP2含量升高(P<0.01,P<0.05)；与模型血清组比较,电针血清组MSCs增殖速度明显加快(P<0.01),Collagen I含量明显降低(P<0.01)、MMP2含量明显升高(P<0.01)。结论：电针“委中”穴加快MSCs增殖,促进腰多裂肌损伤后的修复可能是通过调节细胞外基质中与骨骼肌纤维化有关的Collagen I、MMP2的表达实现的
Objective：To observe the intervention effect of electro-acupuncture (EA) serum at “Weizhong (BL40)” on proliferation of multifidus muscle Satellite Cells (MSCs) injury and expression of extracellular matrix (ECM) components Collagen I, Matrix Metalloproteinases2 (MMP2), so as to discuss the possible mechanism of multifidus muscle regeneration. Methods：25 male Sprague Dawley rats were randomly divided into normal group (NG), model group (MG), EA-BL40 group (EG), every group has 8 rats. Rats in MG and EG were treated with intramuscular injection with 0.5% Bupivacaine at bi-multifidus muscle of lumbar 4 and 5 (4 points, 100μL for every point). Rats in EG was treated with EA at BL40 for 3 day. No EA intervention was given to the rats in NG and MG. Blood samples of the abdominal artery of rats in the 3 groups were separately collected for extracting serum, the serum were inactivated and filtrated, and then were respectively applied to the Dulbecco’s Modified Eagle Media (DMEM) culturing each multifidus muscle MSCs of normal serum, model serum, EA serum for analyzing the EA serum on the proliferation state of MSCs by Cell Counting Kit-8 (CCK-8) and the expression of Collagen I, MMP2. Results：Compared with the normal serum group, the proliferation level and the expression of Collagen I and MMP2 of MSCs in the model serum group were increased (P<0.01, P<0.01, P<0.05), while in comparison with the model serum group, the proliferation level and the expression of MMP2 of MSCs were significantly increased (P<0.01, P<0.01) and the expression of Collagen I was decreased (P<0.01) in the EA serum group. Conclusion：EA-BL 40 can accelerate the proliferation of MSCs and improve the repair of injured multifidus muscle through the ECM components Collagen I and MMP2 that are related to skeletal muscle fibrosis.