目的：探讨肉豆蔻醚（myristicin）对结肠癌细胞增殖、迁移和侵袭的作用及机制。方法： MTT法检测肉豆蔻醚在体外对结肠癌细胞HCT116和LOVO增殖的影响； Annexin V/PI双染法检测肉豆蔻醚对HCT116和LOVO细胞凋亡的影响；划痕试验和Transwell实验分别检测肉豆蔻醚对HCT116和LOVO细胞迁移和侵袭的影响；western blot实验检测肉豆蔻醚对MEK1/2、ERK1/2、CyclinD1、E-cadherin、MMP-2以及MMP-9表达的影响。动物荷瘤模型检测肉豆蔻醚在体内对HCT116增殖和凋亡的影响。结果：肉豆蔻醚作用后，HCT116和LOVO细胞增殖、迁移和侵袭受到显著抑制，细胞出现显著凋亡；肉豆蔻醚作用后，HCT116和LOVO细胞内MEK1/2、ERK1/2、CyclinD1、MMP-2以及MMP-9蛋白表达水平显著降低，而E-cadherin蛋白表达水平显著增高。动物实验表明，肉豆蔻醚可以显著的抑制HCT116裸鼠移植瘤的增殖，诱导肿瘤凋亡。结论：本研究发现肉豆蔻醚在体内体外均能发挥抑制结肠癌细胞生长的作用，为临床用药提供一定的实验依据，为肿瘤治疗提供新的靶点和策略。
Objective: To investigate the effect and molecular mechanism of myristicin on proliferation migration and invasion of colon cnacer cell lines (HCT116 and LOVO). Methods: the effect of myristicin on proliferation of colon cancer cells HCT116 and LOVO in vitro was detected by MTT assay; The apoptosis of myristicin on HCT116 and LOVO cells was detected by Annexin V/PI; The migration and invasion ability of myristicin were detected by wound healing and Transwell assay; The effect of myristicin on the expression of MEK1/2, ERK1/2, CyclinD1, E-cadherin, MMP-2 and MMP-9 was analysised by Western blot; The effect of myristicin on the proliferation and apoptosis of HCT116 in animal model of tumor-bearing. Results: The proliferation, migration and invasion of HCT116 and LOVO cells were significantly inhibited by myristicin. After myristicin treatment, the expression of MEK1/2, ERK1/2, CyclinD1, MMP-2, and MMP-9 were significantly decreased in HCT116 and LOVO cells, while the expression of E-cadherin protein was significantly increased. Animal experiments showed that myristicin could significantly inhibit the proliferation of xenografts in nude mice and induce tumor apoptosis. Conclusion: Myristicin could inhibit the growth of colon cancer cells both in vitro and in vivo, which provided a certain experimental basis for clinical drug use, and provided new targets and strategies for cancer treatment.