蛴螬  ; 激光  ; 血－视网膜屏障（BRB）  ; 神经营养因子受体（TrkC、 NGF）

【】目的：探讨蛴螬促进激光损伤兔血－视网膜屏障后的修复作用及机理。方法：30只成年健康有色家兔随机分为正常对照组、模型对照组、蛴螬组。采用激光损伤血－视网膜屏障动物模型,在屏障损伤后1周及2周两个时相,观察蛴螬对兔血－视网膜屏障损伤修复情况、血－视网膜屏障组织形态结构及神经营养因子受体TrkC、 NGF表达及影响。结果：蛴螬能促进损伤的血－视网膜屏障修复,减轻激光导致的视网膜组织及细胞形态学损伤,促进TrkC、 NGF的表达,抑制视网膜细胞增殖与变性,从而改善视网膜功能。结论：蛴螬可能通过改善视网膜组织能量代谢,清除氧自由基,促进神经营养因子受体的表达,抑制视细胞增殖,从而起到保护血－视网膜屏障组织结构,促进损伤后功能恢复的作用。

Grub  ;  laser  ;  blood-retinal barrier (BRB)  ;  neurotrophin receptor (TrkC, NGF)

【】Objective: To explore the role of sputum in promoting the repair of laser-damaged rabbit blood-retinal barrier and its mechanism. Methods: Thirty adult healthy colored rabbits were randomly divided into normal control group, model control group and sputum group. Laser-damaged blood-retinal barrier animal model was used to observe the repair of blood-retinal barrier damage, blood-retinal barrier tissue morphology and neurotrophin receptor TrkC in rabbits at 1 week and 2 weeks after barrier injury. NGF expression and influence. Results: It can promote the repair of blood-retinal barrier damage, reduce the retinal tissue and cell morphology damage caused by laser, promote the expression of TrkC and NGF, inhibit the proliferation and degeneration of retinal cells, and improve the retinal function. Conclusions: It may improve the energy metabolism of retinal tissue, scavenge oxygen free radicals, promote the expression of neurotrophin receptors, inhibit the proliferation of visual cells, thereby protecting the structure of blood-retinal barrier and promoting the recovery of function after injury.

国家自然科学基金资助项目(81804150)；湖南省自然科学基金资助项目(06JJ50056)；湖南省科技厅科研基金资助项目(02ssy3096)；湖南省中医药科研基金资助项目(2010012,201438),湖南省教育厅科研基金资助项目(09C729),湖南中医药大学科研基金资助项目(3601010157)；中医药防治五官科疾病湖南省重点实验室建设项目(2017TP1018)；中医药防治眼耳鼻咽喉疾病与视功能保护湖南省工程技术研究中心建设项目(2018TP2008)；长沙市科技计划项目(K1501014-31,kc1704005)；国家中医药管理局中医眼科学重点学科建设项目(ZK1801YK015)；湖南省中医五官科学重点学科建设项目。