目的 研究益气活血中药单体人参皂苷Re联合川芎嗪（TMP）对间歇性低氧（IH）复合胰岛素抵抗（IR）的3T3-L1脂肪细胞模型的干预效应，并观察SREBP-1c/FAS信号通路在其中的作用。方法 实验采用IH复合IR的3T3-L1脂肪细胞模型，分别以人参皂苷Re及TMP作为干预药物，首先进行中药最优配伍浓度体外筛选，然后选择最优配伍浓度作为干预剂量，观察其对模型细胞SREBP-1c/FAS信号通路的干预效应。结果 人参皂苷Re 1 μmol·L-1联合TMP 10 μmol·L-1时，对IH条件下SREBP-1c、FAS mRNA表达水平抑制最明显。该单体配伍及Betulin能抑制IH引起的SREBP-1蛋白表达水平上调，升高IH条件下IRS-1、SOD mRNA表达水平，降低IH条件下HIF-1α mRNA表达水平。结论 人参皂苷Re与TMP体外联合作用能抑制IH对IR-3T3-L1细胞SREBP-1c/FAS通路表达水平的上调作用，且有一定的改善IR、氧化应激的效应。
Objective To explore the interventional effects of ginsenoside Re combined with Ligustrazine (TMP) on intermittent hypoxia (IH) composite insulin resistance (IR) cell model of 3T3-L1 adipocytes in vitro, and to observe the effect of SREBP-1c/FAS signal pathway. Methods IH composite IR cell model of 3T3-L1 adipocytes was built. The effective components of Chinese medicine of supplementing Qi and activating blood circulation including ginsenoside Re combined with ligustrazine (TMP) were used as intervention drugs. The optimal compatibility concentration of them was screened in vitro, which was selected as the intervention dose to observe the effect on SREBP-1c/FAS signaling pathway in IH composite IR 3T3-L1 adipocytes model. Results The combination the concentration of Re 1 μmol·L-1 with TMP 10 μmol·L-1 inhibited the mRNA expression of SREBP-1c and FAS after IH treatment. The drug inhibited IH-induced upregulation of protein expression of SREBP-1 and FAS, increased the mRNA expression level of IRS-1 and SOD and reduced the mRNA expression level of HIF-1α after IH treatment. Conclusion The study demonstrates the combination of ginsenoside Re and TMP has the effect of inhibiting expression of IH-induced SREBP-1c/FAS pathway in IR-3T3-L1 adipocytes and may have the effect of improving IR and oxidative stress.